In control group, inflammatorry cells were abundant and completely filled the gap of wound. The severity of the reaction in propolis group was lower severe than SS and control group. Table 1: Changes in wound sizes of three groups during the healing periods (n=10).Īt the 10th day of experiment, histopatologically there was infilammatory reaction including lymphocytes, macrophages and neutrophiles and fibroblastic loose connective tissue in dermis of all groups examined. The wound contraction and wound healing rates of propolis were higher than treatment and control groups (Figure 1), (Table 1).įigure 1: The wound healing rates of propolis (p)were higher than treatment (ss) and control (c) groups. All wound sites was healed completely 17 days after surgery. Macroscopically, in all treatment groups, no difference was detected with regard to inflammatory response heat, redness, and swelling. No mortality was seen in the animals during the study. The samples were fixed in 10% neutral buffered formalin, and were cut into 5 µm sections and stained with hematoxylin and eosin (H&E), and examined microscopically 9. Ten and 20 days later, the rabbits were anaesthesied and the skin tissue biopsy samples were collected from rabbits for histopathological examinations. The result were considered as significant P≤0.05. The statistical comparison was made with ANOVA. During the examination, the day wound contraction started, the rate of wound contraction, the day epithelization and the number of days in which wound fully completed were evaluated. Briefly area (cm 2) = P/K.M, where P is the value of a particular wound site obtained on the PB, K is a rate constant for expressing the value of the PB as cm 2, and M is magnitude of tracings after scanning. The data obtained were calculated as described by Kilic et al. The wound boundaries were traced on a transparent sheets were scanned and the areas of wound sites and epithelization fronts were measured with the help of the paint-brush (PB) computer program. A wound site was considered macroscopically to be fully healed when its whole surface was covered with epithelium. These applications were repeated every day. These wound sites were allocated to three groups and covered with 50% propolis cream (Turkish propolis), silver sulfadiazine skin cream and bepanthane cream (control), respectively. On each animal, two cranially and one caudally located square (4 cm') full-thickness skin wounds were created using a template prepared from an X-ray film. All rabbits were anesthetized with intramuscular administration of 10 mg/ kg xylazine hydrochloride (Rompun, Bayer) and 50 mg/ kg ketamine hydrochloride (Ketanes-AIke). The dorsal aspects of 5 male and 5 female rabbits were clipped and prepared for aseptic surgery.
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